More than just another dynamic light scattering instrument.
Unique Cuvette Based DLS
The DynaPro® NanoStar® is a unique cuvette-based DLS instrument used for the analysis of proteins, promiscuous inhibitors, micelles, quantum dots, liposomes, metallic nanoparticles and more. To accommodate scarce and precious samples, specially designed quartz cuvettes afford robust, accurate measurements with as little as 1 µL of solution, while our proprietary disposable cuvettes provide comparable sensitivity with as little as 4 µL!
Compatible with DYNAMICS
The NanoStar is accompanied by DYNAMICS, our powerful software package for analyzing batch dynamic light scattering measurements. The Security Pack (DYNAMICS SP) option provides 21 CFR (11) compliance for GMP/GLP use.
Coupled with the versatile and user-friendly DYNAMICS software, the NanoStar may be utilized to measure not just size, size distributions, particle concentration and weight-average molar mass, but also solution properties relevant to sample stability: the second virial coefficient (A2 or B22), the diffusion interaction parameter (kD or D1, closely related to the second virial coefficient), and the temperature of protein melting or aggregation onset (TM, Tonset).
Cuvette, and yet...
... still another feature unique to the NanoStar: the optical fiber pickup may be coupled to any of Wyatt's on-line MALS detectors - the DAWN® or miniDAWN® - to add on-line DLS measurements in the same detection volume as SEC-MALS or FFF-MALS measurements.
Combining numerous benefits, the NanoStar is an unparalleled cuvette-based DLS detector. For higher productivity, consider the DynaPro Plate Reader, the only commercially available DLS plate reader measuring in situ for high-throughput screening of thousands of conditions per day in industry-standard microwell plates.
- Rh from 0.18 nm - 2500 nm
- Temperature from -15°C to +150°C
- Molecular weight from 300 Da to 1 MDa
- Separate, optimized SLS and DLS detection channels
- Auto-attenuation over five orders of magnitude
Versatile, High-Quality Options
Make measurements in super-high-quality quartz cuvettes with as little as 1.25 µL of sample, aqueous or organic solvents, over the entire, extended temperature range. This cuvette incorporates a solvent reservoir to prevent sample evaporation over high-temperature or extended analyses.
Our proprietary disposable cuvettes nearly match the optical quality of quartz yet require only 4 µL of sample, aqueous or select polar solvents. For convenience, larger-volume quartz and disposable cuvettes are also available.
On-Line MALS and DLS in a Shared Flow Cell
Simultaneous determination of molar mass and size is crucial for structural and conformational analysis of macromolecules (e.g., branching ratio of polymers, discriminating empty or filled vesicles, etc.). For smaller molecules such as proteins, MALS cannot measure size directly via the angular dependence and so DLS is an important complementary technique.
On-Line DLS is a valuable addition to your SEC-MALS or FFF-MALS characterization instrumentation, providing hydrodynamic radii and, in combination with MALS, the shape factor Rg:Rh of larger nanoparticles. Instead of adding another detector in-line, with the associated band broadening, dilution, signal misalignment and loss of resolution, Wyatt's innovative solution is to share the MALS laser and flow cell with the DLS detector.
A NanoStar can be coupled to a MALS flow cell by means of a fiber coupling accessory. The ASTRA chromatography software connects to the MALS and DLS detectors to acquire data simultaneously from both, analyzing and reporting the individual information (molecular weight, rms radius, hydrodynamic radius) and results derived from the combination, such as molecular configuration by shape factor.
Commercially available DLS instruments generally incorporate an avalanche photodiode (APD) as a single photon counting module (SPCM). APD-based SPCMs are excellent and robust for DLS analysis thanks to their rapid, high-gain response; the NanoStar utilizes a temperature-stabilized APD coupled to an advanced double density, multi-tau (DDMT) correlator for analysis of diffusion coefficients, size and size distributions. The DDMT correlator incorporates 512 bins covering time scales of 100 nsec to 1 hour in order to address a huge span of particle sizes - ranging over hydrodynamic radii Rh of 0.18 nm - 2500 nm.
While size is the primary application of DLS, the measurement of molar mass is often of interest as well. The molar mass of macromolecules having a specific conformation (e.g., globular proteins) may be often estimated from the measured Rh. However, this model-based estimation of mass is often incorrect since many molecules of interest do not correspond to these models. In fact, molar mass may be measured directly from first principles by means of static light scattering (SLS).
In addition to the SPCM-based DLS detection channel, the NanoStar incorporates a separate, optimized SLS detection channel featuring a highly linear, low noise photodiode - in fact, the same photodiode used in our on-line multi-angle light scattering (MALS) detectors. This SLS channel guarantees sensitive and accurate molar masses from 300 Da to ~10 MDa (the upper limit depends on the molecular conformation, and the maximum accurate size may be estimated from the measured Rh).
And More Unique Technology
In order to insure that the NanoStar never goes out of range, it comes equipped with automatic attenuation over five orders of magnitude. Its air-launched laser, coupled with proprietary optics for maximum sensitivity, make single-mode optical fiber technology obsolete. The temperature control system in the NanoStar is unequalled in comparable dynamic light scattering detectors, providing rapid temperature ramps with an overall temperature range of -15°C to +150°C.
DYNAMICS - Automates well-plate scans, temperature ramps, etc. and analyzes DLS data to determine size distributions, temperature onset of melting or aggregation, and the diffusion interaction parameter kD.
|Size Range (Radius)||0.2 to 2500 nm|
|Static Scattering Molecular Weight Range||300 Da to 1 MDa (sample dependent - maximum molecular radius ~ 12 nm)|
|Minimum Concentration (lysozyme) *||0.1 mg/mL|
|Laser Wavelength||658 nm; 785 nm optional|
|Laser Power Control||Programmable 10% to 100%|
|Scattering Angle Detected||90°|
|Sample Cell Options||
1.25 µL quartz cuvette
4 µL disposable cyclic olefin copolymer (COC) cuvette
standard 1 cm cuvettes with adapter
-15°C to +150°C †
Dry air or nitrogen required for operation below 20°C
|Data Acquisition Time||1 to 3600 s|
|Correlator||512 channels, multi-tau, 100 nsec sampling time|
|PC ports||Ethernet connectivity|
|Dimensions||60 cm (L) x 36 cm (W) x 18 cm (H)|
|Weight||13.5 kg (29.8 lbs)|
* 14 kDa Lysozyme, PBS, 100 seconds data acquisition.
† Absolute accuracy of ± 0.3°C.
Host PC requirements may be found in Computer Requirements.
Specifications subject to change without notice.
"Great product, fantastic service, amazing company"
"The machine is excellent for sample characterization and intermolecular interaction assays (we work on RNA and protein interactions). The service engineers and Wyatt corporate have been outstanding and have bent over backwards to help our newly established laboratory with software, hardware, and user issues." Andrea Berman, University of Pittsburgh
"I would recommend this instrument as must have for full protein characterization"
"We are using this instrument to characterize our protein product. It is an essential tool for analytical and formulation to make decision on the go/no go project." Luis Montrond, Takeda Pharmaceutical
"Great and simple result giving interface"
"Great instrument giving clear results in short time. Temp controlling makes it easy to estimate temp for crystallization and potential protein aggregation. Kd analysis helps to modify ionic strength of the buffers. Connection easy to set up to computer in case your computer crashes. Wyatt staff very helpful in troubleshooting and analysis of results. After sale care is very good. You will not be left alone after warranty expires." Michal Boniecki, University of Saskatchewan
"Very user friendly, high-throughput size measurements"
"Very easy to use, you just enter a few values and make a spreadsheet with sample IDs, then press 'Go'. When you come back you have a plate full of data." Tim Ruckh, Verily
Other DLS & ELS Instruments
DynaPro® Plate Reader - The only commercially available instrument that measures DLS and SLS, for size and molar mass, directly in situ in standard 96, 384 or 1536 microwell plates. The non-perturbing DynaProPlate Reader offers integration with robot liquid handlers and multi-technique plate-based assay protocols. Temperature-controlled over 4°C to 85°C.
Mobius - The finest, most versatile light scattering instrument for reliable electrophoretic mobility and size measurements of proteins, polymers, and all types of nanoparticles. Wyatt Technology's Mobius is the first and only light scattering instrument that makes reliable, reproducible, and non-destructive electrophoretic mobility measurements of macromolecules as small as 2 nm in radius (as well as micron-size nanoparticles) in most aqueous buffers.