Available in two formats, Asymmetric Flow Field-Flow Fractionation (AF4) and Hollow-Fiber Flow Field-Flow Fractionation (HF5) provide numerous advantages over other separation techniques:
- Fractionate macromolecules and nanoparticles 1 nm to > 1 µm
- Eliminate column shear that may degrade fragile samples
- Avoid adverse interactions with column packing beads
FFF-MALS analysis of whole serum to determine mass and size distributions of all components.
Coupling an FFF system to a set of Wyatt MALS and/or DLS detectors creates a powerful system for accurate and robust characterization of molar mass and size distributions for simple or complex samples:
- Liposomes, virus-like particles or inactive viruses
- Whole serum
- Nanoparticles and biopolymers in the food industry
- Environmental nanoparticles
- Heterogeneous biopolymers and synthetic polymers
- Oligomers and aggregates
- Conjugates such as ADCs, PEGylated proteins or block co-polymers
FFF can also be coupled to ICP-MS for elemental analysis.