Quantifying Viral Vector Attributes with Light Scattering

Michelle Chen

Presented By: Michelle Chen, Ph.D., Wyatt Technology
Presented Live: March 24, 2020

Viral vectors, such as adeno-associated virus (AAV) and lentivirus (LV), are widely used in gene and cell therapies. In order to ensure the safety and efficacy of these emerging therapeutics, it is critical to fully characterize the viral vectors throughout the development process, as well as quantify their critical quality attributes (CQAs) across manufacturing and quality control.

In this webinar, we discuss how dynamic light scattering (DLS) and multi-angle light scattering combined with size exclusion chromatography (SEC-MALS) or field-flow fractionation (FFF-MALS) characterize AAV, LV, and other gene delivery vectors. Biophysical properties and attributes covered by these techniques include size, concentration, aggregation, stability and capsid content.

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