Wyatt Technology provides the essential techniques for characterizing macromolecules and nanoparticles in solution, to determine molar mass, size, charge, and interactions.
 

Solutions

What's the solution to characterizing your macromolecules or nanoparticles in solution?

Browse these topics to learn more about the physical properties measured, experimental techniques supported, classes of analytes studied or the users served by Wyatt instruments.

Molar Mass or Molecular Weight

Multi-Angle static Light Scattering (MALS) measures molar mass directly, in solution. Combined with a fractionation technique like Size Exclusion Chromatography (SEC) or Field-Flow Fractionation (FFF), MALS determines absolute molar mass distributions—independent of elution time and as the molecule exists in solution.

Size

The size of macromolecules or nanoparticles is measured via two light scattering methods: MALS and Dynamic Light Scattering (DLS). Each technique contributes critical pieces of the characterization puzzle.

Charge/Zeta Potential

Molecular charge and nanoparticle zeta potential are core properties of a solute and a solvent, relevant to colloidal stability, chemical modifications and other key qualities.

Interactions

Biomolecular interactions are key to understanding a host of phenomena ranging from structure-function relationships and biotherapeutic activity through stability and aggregation.

Conformation

Shape, structure or conformation of macromolecules and nanoparticles are analyzed quantitatively via MALS, differential viscometry, and DLS.

Conjugation

The combination of MALS, UV and refractive index (RI) detection with SEC, plus ASTRA's Conjugate Analysis algorithm, offers the solution to the characterization of conjugated macromolecules.

SEC-MALS

Conventional Size Exclusion Chromatography (SEC) relies on reference standards that do not accurately represent your molecules. Multi-Angle static Light Scattering (MALS) measures molar mass directly, independent of elution time, so you can have confidence in the values you report.

FFF-MALS

Field-Flow Fractionation (FFF) is a versatile separation technique that overcomes many of the limitations of analytical SEC. Combine with MALS and/or Dynamic Light Scattering (DLS) for absolute molar mass and size.

CG-MALS

Composition-Gradient, Multi-Angle static Light Scattering (CG-MALS) is a powerful technique for characterizing a wide range of biomolecular interactions, label-free and immobilization-free. CG-MALS determines binding affinity, absolute stoichiometry, equilibrium and kinetics of simple or complex interactions.

DLS

Widely used in biochemistry, biotechnology and pharmaceutical development, Dynamic Light Scattering (DLS) is popular wherever the size and size distributions of macromolecules and nanoparticles need to be measured quickly and easily. Wyatt offers high-throughput, automated DLS as well as conventional formats.

MP-PALS

Massively Parallel Phase Analysis Light Scattering (MP-PALS) improves over conventional Phase Analysis Light Scattering to determine electrophoretic mobility and charge with maximum sensitivity and minimal damage to fragile biomolecules.

SEC-IV

Intrinsic Viscosity in conjunction with Size Exclusion Chromatography (SEC-IV) characterizes those classes of polymers that are incompatible with light scattering, and extends MALS conformational analysis to low molecular weight samples.

Biotherapeutics

Light scattering technologies assist at each stage of the biotherapeutic R&D pipeline, with uniquely versatile technologies for biophysical screening and characterization, from target and candidate discovery to selection, optimization, purification, and formulation.

Proteins

Proteins conjugates, aggregation, colloidal stability and protein-protein interactions can be analyzed with MALS, high-throughput Dynamic Light Scattering (DLS), and Composition-Gradient, Multi-Angle static Light Scattering (CG-MALS).

Biopolymers

MALS coupled to Size Exclusion Chromatography (SEC-MALS) or Field-Flow Fractionation (FFF-MALS) is the most robust and reliable method for analyzing heterogeneous distributions typical of biopolymers.

Synthetic Polymers

Branching and conformational analyses of synthetic polymers via MALS, Dynamic Light Scattering (DLS), and Field-Flow Fractionation coupled with MALS (FFF-MALS).

Nanoparticles

Size, composition, mass and zeta potential of nanoparticles can be analyzed via MALS, Dynamic Light Scattering (DLS), Field-Flow Fractionation (FFF) and Electrophoretic Light Scattering (ELS).

Biopharmaceutical

Light scattering is used by biopharmaceutical companies in their R&D, Formulations, QC, and Manufacturing settings in compliance with FDA regulations.

Chemical

Global chemical companies rely on light scattering instrumentation to standardize worldwide characterization techniques for natural and synthetic rubbers, olefins and other petrochemical products.

Medical Device

Wyatt's cutting edge technology has assisted R&D scientists worldwide to better characterize the molecular properties of materials that make up various implantable medical devices.

Academic

Wyatt light scattering instruments are used for teaching and research at some of the most prestigious academic centers in the world.

Government

Wyatt instruments are used in U.S. government research laboratories, including the Centers for Disease Control (CDC), the National Institutes of Health (NIH) and various national laboratories affiliated with the Department of Energy