Biotherapeutics Application Notes

Aggregation, conjugation, formulation, and interaction in drug development.

SEC-MALS
SEC-MALS-DLS
SEC-MALS-VI
µSEC-MALS
FFF-MALS
FFF-MALS-DLS
CG-MALS
DLS
ELS
Batch MALS

White Papers
1
Aggregate Characterization by Orthogonal Light Scattering Methods [poster]
2
E-book: Advancing biopharma analysis with light scattering

Application Notes
1
AN1301: PLGA Molar Mass and Conformation by SEC-MALS-IV
2
Polysaccharides: Polyvalent Pneumococcal Vaccine

3
Polysaccharides: Low Molecular Weight Heparins Used as Anticoagulants
4
Polysaccharides: Hyaluronic Acid

5
AN1603: Protein Reagent Quality for Drug Discovery
6
Protein-Protein Interactions of Tumour Suppressor Proteins

7
Antibody-Drug Conjugate (ADC) Analysis
8
AN1605: Identification of Insulin Oligomeric States

9
AN6301: Discriminating Heparin from Chondroitin Sulfate by Charge:Mass Ratio
10
AN1302: Hyaluronic Acid MW and Conformation by SEC-MALS-IV

11
Stabilization of a Protein by a Peptide Mini-Chaperone [poster]
12
Aggregation of a Recombinant Protein: Comparison of SEC-MALS, SEC-QELS and Plate-Based DLS [poster]

13
Vaccines: Forced Aggregation of Antigens [poster]
14
Stability of Enzyme Quaternary Structure [poster]

15
Amyloid-β Derived Diffusable Ligands [poster]
Application Notes
1
Aggregation of a Recombinant Protein: Comparison of SEC-MALS, SEC-QELS and Plate-Based DLS [poster]
2
Stability of Enzyme Quaternary Structure [poster]
Application Notes
1
AN1301: PLGA Molar Mass and Conformation by SEC-MALS-IV
2
AN1302: Hyaluronic Acid MW and Conformation by SEC-MALS-IV
Application Notes
1
High Speed Process Monitoring with microSEC-MALS
White Papers
1
Aggregate Characterization by Orthogonal Light Scattering Methods [poster]
2
Nanoparticle Drug Delivery Characterization by FFF-MALS-DLS

Application Notes
1
Vaccines: Recombinant Adenovirus
2
Monoclonal Antibodies and the Preparative Use of Asymmetric Flow Field-Flow Fractionation (AF4)

3
Endotoxin-Free Separation of Protein Species
4
Botulism Antitoxin Characterization

5
IgG Aggregation via Fluorescent Dye Detection
6
Separation of rh-GCSF

7
A Colloidal Drug Carrier System
8
AN2602: Size Distributions of Liposomes and Virus Particles by FFF-MALS

9
Vaccines: Characterization of Virus-Like Particles by Asymmetrical Flow Field-Flow Fractionation
10
Peptide Hormone Aggregation

11
Vaccines: Virus Particle Characterization
12
Quantification of Insoluble Monoclonal Antibody Aggregates

13
Separation of GCSF using AF4
14
Virus-Like Particle Characterization by AF4

15
Vaccines: VLPs Packed with DNA Payload [poster]
16
Vaccines: Protein-Polysaccharide Conjugates [poster]

17
Polysaccharides: Separation of Hyaluronic Acid on Frit-Inlet FFF-MALS [poster]
18
Silver Nanolipids [poster]

19
Dendrimers [poster]
20
Aggregation of Antibody-Drug Conjugates [poster]

21
Zeta Potential vs Size Distributions by Flow MP-PALS
White Papers
1
Nanoparticle Drug Delivery Characterization by FFF-MALS-DLS
Application Notes
1
Zeta Potential vs Size Distributions by Flow MP-PALS
White Papers
1
Aggregate Characterization by Orthogonal Light Scattering Methods [poster]
2
E-book: Advancing biopharma analysis with light scattering

Application Notes
1
Self-Associating Antibody Solutions at High Concentrations
2
Self-Association of Insulin: Affinity and Stoichiometry

3
Competitive Binding Between Thrombin, Antithrombin III, and a Monoclonal Anti-Thrombin Antibody
4
Binding of Thrombin-α and an Anti-Thrombin Antibody

5
Kinetics of Covalent Thrombin-Antithrombin Association
6
Lot-to-Lot Comparability Studies of Monoclonal Antibodies

7
Antibody-Antigen Binding
8
PEG-Protein Interactions for Stable Formulations [poster]

9
Understanding Antibody and Viral Glycoprotein Interactions
White Papers
1
AN6001: Charge and Interaction Analysis for Predicting Antibody Formulation Stability
2
The Diffusion Interaction Parameter kD as an Indicator of Colloidal and Thermal Stability

3
Characterizing Stable Protein Formulations
4
Aggregate Characterization by Orthogonal Light Scattering Methods [poster]

5
Protein Quality Control in SPR and BLI High-Throughput Screening Studies
6
High Throughput Dynamic Light Scattering for Screening Biotherapeutic Formulations

7
Nanoparticle Drug Delivery Characterization by FFF-MALS-DLS
8
E-book: Advancing biopharma analysis with light scattering

9
High-Throughput DLS for Biotherapeutics Development
Application Notes
1
Bacterial Endotoxin Interaction with Human Plasma Proteins
2
Nanoscale Aggregation Properties of Neuroprotective Carboxyfullerene

3
Thermal Stability as a Function of pH and Concentration
4
Screening High-Concentration Protein-Protein Interactions

5
Verification of Protein Aggregate Removal
6
AN5001: High-Throughput Screening of TiO2 Nanoparticle Dispersions in Cell Culture Media

7
Surfactants as Chaotropic Agents in Protein Systems
8
Conformation of Trimeric Form of Migration Inhibitory Factor (MIF)

9
Insulin Quaternary Structure as a Function of pH
10
pH Dependent Monomer-Dimer Equilibrium Constants for Glycinamide ribonucleotide transformylase (GART)

11
Monitoring Thermal Stability - Glycosylated Hemoglobin Melting Point
12
Thermal Dissociation and Denaturation

13
High-Throughput Protein Buffer Optimization
14
Aggregation of a Recombinant Protein: Comparison of SEC-MALS, SEC-QELS and Plate-Based DLS [poster]

15
Vaccines: Forced Aggregation of Antigens [poster]
16
Aggregation of Antibody-Drug Conjugates [poster]

17
Vaccines: Time-Resolved Structural Changes and Aggregation in Influenza Virus [poster]
18
Characterization and Formulation Screening of mAbs and ADCs by High-throughput DLS
White Papers
1
Computation of Protein Net Charge from Electrophoretic Mobility
2
AN6001: Charge and Interaction Analysis for Predicting Antibody Formulation Stability

Application Notes
1
Automated Measurements of Electrophoretic Mobility
2
Automated Electrophoretic Mobility Measurement of High Salt Solutions

3
AN6301: Discriminating Heparin from Chondroitin Sulfate by Charge:Mass Ratio
4
Mobility of a Monoclonal Antibody: Comparison of Techniques [poster]

5
Zeta Potential vs Size Distributions by Flow MP-PALS
Application Notes
1
Automated Second Virial Coefficient Determination Using a Binary HPLC Pump
2
Automated, Low-Volume Second Virial Coefficient Measurement

3
Vaccines: Time-Resolved Structural Changes and Aggregation in Influenza Virus [poster]