Separation and Characterization of Macromolecules with AF4-MALS

Presented By: Pernille Harris, Ph.D., and Esben Thormann, Ph.D., Denmark Technical University
Presented Live: December 10, 2019

Multi-angle light scattering (MALS) is an absolute technique for determining the molar mass of macromolecules in solution. While SEC-MALS is the most common method for utilizing MALS with peptides, proteins and polymers, size-exclusion chromatography (SEC) is not appropriate for separating all types of macromolecules.

This webinar will present the use of asymmetric-flow field-flow fractionation coupled to MALS (AF4-MALS) for characterizing macromolecules that are not amenable to good separation with SEC.

Two case studies will be presented: GLP-1 analogue oligomers, and polyelectrolytes. An introduction to the GLP-1 analogues, polyelectrolytes and the AF4-MALS technique will be provided as well.

Q&A Webinar Q & A