Proteins Application Notes

Molar masses and sizes, stoichiometry of proteins and biomolecular interactions, and electrophoretic charge.

SEC-MALS
SEC-MALS-DLS
IEX/RPC-MALS
UHPLC-SEC-MALS
FFF-MALS
FFF-MALS-DLS
CG-MALS
DLS
ELS
Batch MALS

Proteins Application Notes

White Papers

WP1621: Multi-Attribute Quantitation of Antibody-Drug Conjugates (ADCs) by LC MALS

WP3001: Characterizing protein-DNA interactions

WP1618: Enhanced SEC-MALS analysis of therapeutic mAbs, biosimilars and AAVs with XBridge Premier columns

Aggregate Analyses by Light Scattering – Complementary and Orthogonal [poster]

WP1615: SEC-MALS for absolute biophysical characterization

Overcoming Protein Fluorescence in MALS Measurements

Application Notes

AN1622: SEC-MALS Analysis of Proteins with Empower™ Chromatography Data System

AN1620: Development of a Platform SEC-MALS Method for Therapeutic Monoclonal Antibodies

AN1619: Binding Stoichiometry of an Antibody Fragment

AN1614: Multi-protein complexes identified by SEC-MALS

Crystalline Eye Lens Proteins: Conformational Changes

AN1610: Stoichiometry of Intrinsically-Disordered Protein Complexes

Next Steps in AAV Characterization via Light Scattering Measuring AAV Critical Quality Attributes [poster]

AN3002: Understanding Antibody and Viral Glycoprotein Interactions

Stabilization of a Protein by a Peptide Mini-Chaperone [poster]

Stability of Enzyme Quaternary Structure [poster]

On-Line MALS-DLS Characterization of Proteins with Unusual Elution Properties

Mega-Dalton Protein Complexes

Low Molecular Weight Lens Peptides and Changes in the Oligomeric Organization of αβ-crystallin

AN1603: Protein Reagent Quality for Drug Discovery

Amyloid-β Derived Diffusable Ligands [poster]

eBook: Advancing HPLC/ UHPLC Analysis with Multi-Angle Light Scattering Technology

Stoichiometry of Lysin Holoenzyme

Role Of Oligomeric Size in the Chaperone Function of αB-Crystallin

Ribosomal Subunits in Protein Biosynthesis

Response Regulator Activation

Protein-Protein Interactions: Scaling-up a Purification Protocol

PEGylated Proteins

Oligomerization and Stability of an AAA-ATPase from Acidinus Two-Tailed Virus

Membrane Proteins – dRI determination of detergent concentration

Membrane Protein Quaternary Structure Analysis and Detergent Selection

Hemoglobin – Unusual SEC Elution Properties

Heat Shock Proteins – Temperature Dependence

Gluten Proteins

Determination of the Stoichiometry of Protein-Protein Complexes

DARPins Complex Characterization

Conjugate Analysis: Lipid Transport Proteins [poster]

Calsequestrin, a Calcium Storage Protein

AN7301: Molar Mass and Size of Globular and Glycoproteins by SEC-MALS-QELS

AN1616: SEC-MALS method for characterizing mRNA

AN1613: Low-Molar-Mass Peptide Characterization by SEC-MALS

AN1608: Transient Protein Self-Association

AN1607: Kinase Fragment Oligomerization

AN1605: Identification of Insulin Oligomeric States

AN1602: Lipid/Membrane Protein Complexes

AN1601: Absolute Molar Mass in UHPLC

White Papers

Application Notes

Stability of Enzyme Quaternary Structure [poster]

On-Line MALS-DLS Characterization of Proteins with Unusual Elution Properties

Mega-Dalton Protein Complexes

Low Molecular Weight Lens Peptides and Changes in the Oligomeric Organization of αβ-crystallin

Role Of Oligomeric Size in the Chaperone Function of αB-Crystallin

DARPins Complex Characterization

Conjugate Analysis: Lipid Transport Proteins [poster]

AN7301: Molar Mass and Size of Globular and Glycoproteins by SEC-MALS-QELS

White Papers

WP1621: Multi-Attribute Quantitation of Antibody-Drug Conjugates (ADCs) by LC MALS

WP8003: IEX-MALS for protein separation and characterization

Application Notes

AN8004: Coupling MALS with preparative ionic-exchange (pIEX) for structural biology applications

Application Notes

eBook: Advancing HPLC/ UHPLC Analysis with Multi-Angle Light Scattering Technology

AN1601: Absolute Molar Mass in UHPLC

White Papers

WP9003: VLP Characterization with the Light Scattering Toolbox

Aggregate Analyses by Light Scattering – Complementary and Orthogonal [poster]

Application Notes

AN2005: Characterizing size distributions of broadly heterogeneous proteins with FFF-MALS

Next Steps in AAV Characterization via Light Scattering Measuring AAV Critical Quality Attributes [poster]

Spongiform Encephalopathies

Vaccines: Protein-Polysaccharide Conjugates [poster]

Separation of GCSF using AF4

Peptide Hormone Aggregation

Monoclonal Antibodies and the Preparative Use of Asymmetric Flow Field-Flow Fractionation (AF4)

Glutenin Protein Polymers

Botulism Antitoxin Characterization