Wyatt Technology

μDAWN

μDawn

 

The First Multi-Angle Light Scattering Detector for UHPLC.

The  μDAWN® can be coupled to any UHPLC-SEC system to determine absolute molecular weights and sizes of polymers, peptides, or proteins directly, without resorting to column calibration or reference standards. The  μDAWN produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC, while meeting or exceeding the sensitivity of the miniDAWN.

  Download our Application Note on the μDAWN


Future-proof your lab! The easy-to-install HPLC Compatibility Kit adapts the μDAWN to standard HPLC/GPC flow conditions. When ready to upgrade to UHPLC, just uninstall and replace the the original flow path. See Options below for more details.

  • The µDAWN MALS detector performs absolute characterization of macromolecules eluting from UHPLC, determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size and concentrations. In most respects the µDAWN is similar to the well-established miniDAWN TREOS II, a workhorse MALS instrument used in hundreds of labs around the world for characterizing proteins and other molecules smaller than 50 nm in radius.

    Distinctly from the miniDAWN, the µDAWN is uniquely suitable for use in conjunction with UHPLC Size Exclusion Chromatography ( µSEC-MALS) thanks to its small interdetector dispersion. In some cases it may be used with ion-exchange or other types of chromatography. Regardless of the specifics, the µDAWN produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC.

    • Mw from 200 Da to 10 MDa
    • Sensitivity: 1.0 µg/mL BSA (66.4 kDa)
    • rg from 10 to 50 nm
    • Ultra-stable laser

    The µDAWN and its companion Optilab UT-rEX Refractive Index Detector interface to most standard UHPLC systems. As these detectors are based on the advanced technology of the miniDAWN TREOS and Optilab T-rEX, respectively, users may be confident in the underlying capabilities cited in thousands of peer-reviewed publications.

    Play video to learn more about the μDAWN and UHPLC

     

    uDAWN UHPLC Experimental Setup
  • uDAWN UHPLC Experimental Setup

    The µDAWN will work downstream of all types of UHPLC systems for analysis of molar mass and size distributions (see SEC-MALS), and derivatives such as conformational analysis. For those systems equipped with a UV, RI, fluorescence or other type of concentration detector, the µDAWN accepts up to 4 analog inputs so a 3rd-party concentration signal may be used in MALS calculations of molar mass. The µDAWN is set up downstream of a UV detector and upstream of a RI detector.

    In most cases it is beneficial to connect an Optilab UT-rEX as well as a UV detector. This permits universal concentration detection regardless of fluorophores or chromaphores. The combination of UV and RI signals is utilized in ASTRA's Conjugation Analysis algorithm to characterize binary complexes such as glycoproteins, PEGylated proteins, membrane proteins enveloped in surfactant, or block co-polymers.

  • uDAWN HPLC Kit, accessory

    μDAWN HPLC Compatibility Kit - Reduces backpressure and increases dispersion to match the flow characteristics of HPLC SEC separations, enabling the μDAWN to serve as a standard HPLC MALS detector.

    COMET - Not an option! Every μDAWN includes an embedded COMET for automated ultrasonic flow cell cleaning.

    WyattQELS DLS Module - To determine size and conformation, the WyattQELS measures hydrodynamic radius of eluting macromolecules in the same flowcell as the MALS detectors. This fully integrated module guarantees dispersionless correlation between molar mass, rms radius and hydrodynamic radius.

    Alternatively, connect an external DLS instrument via fiber optic cable - both the DynaPro NanoStar and Möbiuζ can do double duty as batch and online DLS instruments. Since they collect light directly from the MALS flow cell, the mass/size correlation is still dispersionless.

  • Orbit, accessory

    Orbit - The Orbit helps conserve mobile phase by programmatically directing the solvent to either a waste bottle or back to the solvent reservoir.

    Solaris - Prevents growth of bacteria, fungi or algae in solvents with high efficiency and protects your system from down-time due to contamination.

    WISH - The Wyatt Injection System High-Pressure (WISH) triggers ASTRA data collection and is ideal for use in SEC-MALS without an autoinjector. Also used with an Optilab dRI detector for the determination of dn/dc values.

    SysTrack - Convenient shelving for holding multiple detectors in your experimental setup.

    Thermos - A temperature-controlled chamber for holding separation columns.

  • ASTRA - Our comprehensive software solution for MALS and DLS analysis in chromatography, AF4 or batch mode, included with each detector. ASTRA is available in a 21 CFR(11) compliant version and offers additional options such as particle analysis and a research database.

  • MALS Measurements  
       Molar Mass Range 200 Da to 10 MDa *
       Molecular or Particle Size Range
       (Radius, rg)
    10 nm – 50 nm , up to 150 nm with shape-specific models
       Sensitivity 1.0 µg/mL BSA, typical of 70 ng loading on 15 cm UHPLC-SEC column
    Sample Temperature Control Ambient
    Optics  
    Detectors  
       MALS Detectors High-gain, high dynamic range, static light scattering detectors at 3 angles
       Auxiliary Detectors Laser monitor for stabilization feedback;
    Forward transmission monitor to correct signals for absorbing samples and to assess data quality.
       A/D Resolution 24 bit (detector dynamic range > 16,000,000)
    DLS Size Measurement (with WyattQELS module installed)
       Size Range (Radius, Rh) 0.5 to 30 nm, flow rate 0.3 mL/min
    0.5 to 20 nm, flow rate of 0.5 mL/min
    Sample Temperature Control ambient only
    Laser Properties
       Laser Power Control Programmable 10% - 100%
       Wavelength λ = 660 nm (nominal). Each instrument is individually characterized.
    Band Broadening Upstream detector to μDAWN: < 1.5 μL;
    μDAWN to downstream detector: <1.6 μL FWHM @ 0.3 mL/min
    Wetted Materials 316 stainless steel, fused silica, MP35N, PEEK, PTFE
    Integrated Cell Cleaning Embedded COMET flow cell cleaning system is included with every μDAWN
    Instrument Options Embedded WyattQELS for simultaneous DLS measurements
       
    Electronics  
       Analog Inputs 4 differential analog inputs with 24 bit resolution
       Analog Outputs 3 single ended analog outputs -10 V to +10 V
       Other Inputs/Outputs Alarm in, Autoinject contact closure, Recycle in, Autoinject retransmit, Alarm retransmit, Recycle valve power
       Front Panel Display 162.5 mm, 16-bit, high resolution touch screen + physical keypad
       Computer Interface Ethernet
       Processed Data Transmission Rate 73 Hz to 0.2 Hz
    Dimensions 60 cm (L) x 36 cm (W) x 17 cm (H)

    * depending on dn/dc, the sample concentration, and chromatography conditions, this is typical.

    Typical. Actual range depends on the sample.


    Host PC requirements may be found in Computer Requirements.

    Specifications subject to change without notice.

   

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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