The DAWN may be positioned downstream of all types of standard GPC, HPLC or FPLC systems for analysis of molar mass and size distributions (see SEC-MALS), and derivatives such as conjugation and conformation analyses, all included in the ASTRA data collection and analysis software. For those systems equipped with a UV, RI, fluorescence or other concentration detector, the DAWN accepts up to 4 analog inputs so a 3rd-party concentration signal may be used in MALS calculations of molar mass. The DAWN is generally set up downstream of a UV detector and upstream of a RI detector.
In most cases it is beneficial to connect an Optilab T-rEX RI detector to the eluent stream. This permits universal concentration detection regardless of fluorophores or chromaphores. The combination of UV and RI signals, acquired simultaneously, is utilized in ASTRA's Conjugation Analysis algorithm to characterize binary complexes such as glycoproteins, PEGylated proteins, or block co-polymers.
The DAWN may also be connected to an Eclipse FFF system for characterization of macromolecules and nanoparticles from 1 nm to 1 µm via FFF-MALS.
Both SEC-MALS and FFF-MALS will benefit from an additional WyattQELS module for measuring nanoparticle size via dynamic light scattering (DLS). The WyattQELS utilizes the same laser and flow cell volume as MALS detection. Its collection fiber replaces any one of the 18 MALS detectors in the DAWN.
For analysis of natural and synthetic polymers, the ViscoStar II may also be added as well. Differential viscometry is an orthogonal means of determining molar mass and conformation.