AN1622: SEC-MALS Analysis of Proteins with Empower™ Chromatography Data System

Empower^™ software is globally adopted as a compliant-ready Chromatography Data System (CDS) used to submit novel drug data to regulatory agencies. It now integrates data acquisition and processing for certain applications using size exclusion chromatography with multi-angle light scattering (SEC-MALS). This integration bridges the reliability and traceability of Empower Software with the versatility and precision of SEC-MALS. This new capability can streamline the transfer of SEC-MALS methods from development, where the full suite of analyses available in ASTRA^™ software is required, to a simplified workflow for quality control (QC). In this application note, we used Empower 3.10.0 software to characterize the NIST monoclonal antibody (NISTmAb) standard reference material (SRM 8671)¹ and the BEH^™ 200 SEC Protein Standard Mix by SEC-MALS.