AN1608: Transient Protein Self-Association

Domain antibodies (dAbs) are the smallest functional binding units of antibodies, corresponding to the variable regions of either the heavy (VH) or light (VL) chains of human antibodies. Different dAbs can show different degrees of self-association which drives the need for a cheap, quick—yet robust—technique to study self-association behavior in drug discovery. Transient self-association may be evaluated by SEC-MALS to obtain binding affinity of a protein in monomer-dimer dynamic equilibrium. This study outlines the use of SEC-MALS to determine self-association properties and validates the work by comparison with Analytical Ultracentrifugation (AUC).