AN3007: Estimating protein partitioning in multicomponent aqueous two-phase extraction based on CG-MALS measurements

This work presents a new application of osmotic coefficients from CG-MALS measurements. The coefficients are used to predict partitioning behavior and estimate the precipitation affinity of immunoglobulin G (IgG) within a PEG-phosphate aqueous two-phase system (ATPS). Furthermore, the influence of NaCl and (NH4)2SO4 acting as displacement agents on IgG partitioning was investigated.