Vaccines: Characterization of Virus-Like Particles by Asymmetrical Flow Field-Flow Fractionation

Virus Like Particles (VLP) are used for vaccination and immune stimulation. For quality assurance there is a tremendous need for a technique that allows detection and separation of different VLP fractions (fragments, monomers, dimers, trimers, aggregates) within a formulation. GPC, which is often the analytical standard for protein formulations, is not an ideal separation method due to the VLP particle sizes. Thus, Dynamic Light Scattering (DLS) is often used to investigate the quality of VLP formulations.