Characterizing Protein-Protein Interactions via Static Light Scattering: Reversible Hetero-association

The quantitative characterization of reversible protein–protein interactions is fundamental to the elucidation of basic biological function as well as to the development of new medical products. Composition gradient-multiangle light scattering (CG-MALS) employs static light scattering (SLS) to determine stoichiometry and equilibrium association constants of self- and heteroassociations. This is accomplished without recourse to sample modifications such as fluorescent tagging or surface immobilization, and with no restrictions on buffer composition.