Stoichiometry of Lysin Holoenzyme

Lysins are enzymes produced by bacteriophages that lyse the host’s cell wall to allow the release of newly formed phage progeny. The streptococcal C1 bacteriophage lysin, PlyC, is composed from two subunits: a big, 50 kDa, “catalytic” subunit and a small, 8 kDa, “binding” subunit. In this application note, we used size exclusion chromatography coupled with MALS, refractive index and absorbance detection (SEC-MALS/RI/UV) to define the subunit organization of PlyC.