WP1621: Multi-Attribute Quantitation of Antibody-Drug Conjugates (ADCs) by LC MALS

Liquid chromatography combined with multi-angle light scattering (LC-MALS) is a powerful tool for analyzing antibody-drug conjugates (ADCs), providing a robust approach to obtain, in a single run, multiple quality attributes such as aggregation, fragmentation, drug-antibody ratio (DAR), and ADC extinction coefficient. Here, we demonstrate an easy-to-implement platform method for size-exclusion chromatography coupled with MALS detection (SEC-MALS) to analyze ADC quality attributes under native conditions. Orthogonal techniques, such as reverse-phase chromatography (RPC) and hydrophobic interaction chromatography (HIC), offer insight into the distribution of payload variants.