Beyond Batch Methods: High Resolution Nucleic Acid and LNP Insights with Light Scattering Techniques
Presented by: Parker Lee, Ph.D. and Lavelay Kizekai, Ph.D., Waters Corporation
Presented Live: June 18, 2025
Light scattering techniques are transforming the way we quantify and analyze nucleic acid (NA) and lipid nanoparticle (LNP) therapeutics, playing a critical role in development, RNA quality assessment, and quality control. This webinar will explore how combining multi-angle light scattering (MALS) with field-flow fractionation (FFF) or size-exclusion chromatography (SEC) provides high-resolution insights into critical attributes such as size distribution, NA integrity, overall stability, and payload encapsulation. Recent advances in SEC column technology has increased the quality and resolution of separations for complex mixtures of large NA constructs, as well as NA-protein complexes including Cas9 constructs. FFF-MALS allows for gentle, high-resolution separation, making it ideal for analyzing NA delivery systems like LNPs without compromising their integrity. Additionally, dynamic light scattering (DLS) and electrophoretic light scattering (ELS) enable rapid, automatable batch measurements, allowing for screening of factors such as size and surface charge.