Controlling Polysaccharide Molecular Weight through High-Shear Processing
Presented by: Andrew Lees, Ph.D., Fina Biosolutions
Presented Live: April 9, 2025
In this webinar, we will explore the critical role of controlling polysaccharide molecular weight in the production of conjugate vaccines. The session will delve into the use of high-shear processing, specifically the Dyhydromatics® ShearJet® HL60, to achieve precise degradation of polysaccharides. We will discuss how variations in concentration and the number of passes through the high-shear processor impact the final product, characterized using size-exclusion chromatography with multi-angle light scattering (SEC-MALS). This technique ensures a more uniform product with reduced molecular weight and polydispersity, enhancing reproducibility and product quality.