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The Importance of Light Scattering in Biopharmaceutical Formulation Development


Presented Live: November 15, 2018 & May 9, 2019

Speaker: Mark Spears, Ph.D., Fujifilm Diosynth Biotechnologies

Protein formulation development and characterization relies heavily on a set of analytical tools and techniques to accurately assess a molecule’s stability. This toolbox includes a standard set of tools which has come to us through industry wisdom and experience.

Light scattering is increasingly considered a “standard” tool in the set, and it is a core part of Fujifilm’s approach to protein formulation development. We will introduce how various types of light scattering measurements are used in our work and offer several case studies where light scattering data impacted the project direction or was the only way to accurately measure the product.


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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

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