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µSEC-MALS adds Multi-Angle Light Scattering to UHP-SEC for Measurement of Absolute Molecular Weight and Size

 

Presented Live: January 23, 2018

Speakers: Vincent Hsieh, Ph.D.

We describe recent advances in multi-angle light scattering (MALS) instrumentation that bring absolute molar mass and size measurements to microliter-scale ultra-high-performance size-exclusion chromatography (UHP-SEC). µSEC-MALS achieves the highest resolution and sensitivity of any online light scattering system. The novel design combines dynamic light scattering (DLS) with MALS in the same flow cell.
The National Institute of Standards and Technology (NIST) monoclonal antibody standard reference material SRM 8671 is the benchmark molecule against which many biomolecular analysis techniques are assessed and evaluated. We present µSEC-MALS measurements of SRM 8671 as a demonstration of the unique capability of this system to carry out all the usual SEC-MALS-DLS characterization tasks, while preserving the superb resolution and rapid separations of UHP-SEC. These abilities will have far-reaching impacts at every stage of the development and production of biologics and bio-therapeutic formulations.

 

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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