Here we developed a protocol for Asymmetric-Flow Field-Flow Fractionation (AF4) of small extracellular vesicles. This protocol consists of four sections: I. Preparation of small extracellular vesicles (sEVs) from cell culture. II. AF4 fractionation of sEVs. III Online data collection and analysis. IV. Fraction collection, concentrations and characterization. This protocol is associated with our Nature Cell Biology paper: Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric-flow field-flow fractionation.
Zhang H.; Lyden D. Protocol Exchange 2018, doi:10.1038/protex.2018.002