Protein-protein interactions investigated by DLS

Controlling protein-protein interactions is vital for highly concentrated formulations of therapeutic proteins, as they impact stability as well as viscosity. This article presents high-throughput screening of these interactions using the DynaPro® Plate Reader. Rather than a complex, rigorous characterization, the author demonstrated how simple indicators such as the diffusion interaction parameter kD (moderate concentrations up to 12 mg/mL) and the apparent change in hydrodynamic radius R/Rh with concentration (at 150 mg/mL) are useful for understanding behavior. These parameters showed clear trends with pH and ionic strength that were expected based on electrostatic considerations.

Neergard, M. S. G.I.T. Laboratory Journal  1-2/2014. Link to Article.
Direct Download, with permission of G.I.T.

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