AN6301: Discriminating Heparin from Chondroitin Sulfate by Charge: Mass Ratio

Heparin Structure

Characterizing key parameters of different heparin lots requires multiple techniques. Weight-average molar mass measured by multi-angle static light scattering (MALS) and hydrodynamic radius measured by dynamic light scattering (DLS) can be used to characterize size and polydispersity. Massively-parallel phase analysis light scattering (MP-PALS) and DLS are used to calculate the net charge and characterize the purity of the sample.

In this study, we compared heparin from two suppliers, including one lot modified with super-sulfated material.