Wyatt Technology

ILSC 2015 Plenary: Challenges of Maintaining Structure-Function Complexity of Protein Biologics Throughout the Manufacturing Process


Presented Live: November 4, 2015 at the 24th International Light Scattering Colloquium, Santa Barbara, CA

Speaker: Dr. Joseph Arndt, Senior Scientist, Biogen

A detailed knowledge of interrelationships between structure and function is a requirement for the development of biologics. Environmental perturbations from the manufacturing process such as temperature, pH, and solute additives can impact higher order structure and stability in solution. In this talk, a case study will be presented in which a neurotrophic protein was rendered inactive, with significantly reduced viscosity, as a result of a commonly used viral inactivation step using low-pH incubation. The structure, oligomerization state, and dynamics of the native and viral inactivated protein were examined using orthogonal light scattering techniques to understand the underlying origin of the activity loss and change in rheological behavior. These studies highlight the structure-function complexity of protein biologics that must be maintained at all stages of the drug development.




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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

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