AN9010: Characterizing lentiviral and gammaretroviral vectors by batch DLS and FFF-MALS

This note demonstrates the use of batch dynamic light scattering (DLS) and online multi-angle light scattering and dynamic light scattering in combination with field-flow fractionation (FFF-MALS-DLS) for characterizing large viral vectors such as lentivirus and gammaretrovirus. DLS in batch mode rapidly screens particle size distribution, concentration/titer and stability. FFF-MALS-DLS, on the other hand, provides high-resolution size distributions and viral titer along with an in-depth assessment of aggregation and impurities. The details revealed by FFF-MALS-DLS help scientists and process engineers understand lot-to-lot variations and critical manufacturing parameters, enabling production of these novel therapeutic products with consistently high quality.