Discover Tricks and Tips with our Technical Notes

Already a Wyatt customer? Our technical notes provide step-by-step instructions and tips for how to get the most out of your instrumentation:

  • Quick guides for setting up your instrument or using our software
  • Tips for improving performance
  • Best practices for use and care
  • Step-by-step instructions for performing advanced analyses on your data such as the Protein Conjugate Analysis or Branching Analysis
  • Connection guides for various models of UHPLC and HPLC
  • Troubleshooting guides to keep you up and running

You can find technical notes on our Support Center. Simply go to and login or register for an account. It’s free for customers! Technical notes have their own tile.

Clicking on the tile provides a complete list of available technical notes by instrument or software. You can also find technical notes under each instrument or software application. We continually add and update technical notes with the latest information and applications.

New and Updated Technical Notes

New! TN3508 – Quick Start Guide for SEC-MALS Systems
A comprehensive overview of setup, running, and shutdown of (U)HPLC SEC-MALS systems including processing the data in ASTRA using a Wyatt MALS detector, a Wyatt refractive index detector, and an optional UV detector.

New! TN7504 – Turbidity Measurements with the DynaPro NanoStar II
Step-by-step instructions on how to measure sample turbidity (or opalescence) using static light scattering measurements with a DynaPro NanoStar II.

Updated TN6006 – Eclipse and VISION Handbook
This handbook describes typical procedures, workflows, and best practices for operating a Wyatt Eclipse (NEON) with VISION 3 for field-flow fractionation (FFF), a technique for the separation of macromolecules and nanoparticles. Now with numerous updates including information on the new Rinse Mode UI, new workflows for methods, and additional troubleshooting guidance.

Updated TN1006 – Performing Protein Conjugate Analysis in ASTRA
Instructions for performing a protein conjugate analysis in ASTRA to characterize conjugates such as antibody-drug conjugates, copolymers, membrane protein/surfactants, and PEGylated proteins. Updated for ASTRA 8 and including parameters for system validation with thyroglobulin and other tips.