High-Throughput Analytical Light Scattering for Protein Quality Control and Characterization

We present a review of high-throughput techniques for the characterization and quality control of proteins in the course of purification, evaluation, and formulation, based on static and dynamic light scattering. Multi-angle static light scattering (MALS) in combination with rapid, low-volume UHPLC size exclusion chromatography is effective in characterizing key biophysical properties, while dynamic light scattering (DLS) in high-throughput microwell-plate format provides large quantities of data in a short time to screen many conditions, excipients, cell lines, or candidate biotherapeutics.

Some D., Razinkov V. Methods in Molecular Biology 2019, 2025, 335-359. doi:10.1007/978-1-4939-9624-7_16