The role of electrostatics in protein-protein interactions of a monoclonal antibody

Antibody formulation stability is strongly affected by intermolecular interactions. In this article, researchers used static, dynamic, and electrophoretic light scattering to assess molecular contributions to protein-protein interactions, including excluded volume repulsion, electrostatic attraction, and other short and long range forces. The Calypso® and miniDAWN® enabled them to measure the second virial coefficient (A₂ or B₂₂) as a function of pH and ionic strength. The diffusion interaction parameter (k_D) was measured under the same conditions via dynamic light scattering using the DynaPro® Plate Reader. These values were then correlated to the electrophoretic mobility measured by electrophoretic light scattering in the Mobius®.

Roberts, D.; Keeling, R.; Tracka, M.; van der Walle, C.F.; Uddin, S.; Warwicker, J.; Curtis, R. Molecular Pharmaceutics  2014, 11, 2475–2489. doi:10.1021/mp5002334