Aggregate Analyses by Light Scattering – Complementary and Orthogonal [poster]

Size exclusion chromatography (SEC) combined with a multi-angle light scattering (MALS) and a concentration detector (either UV or differential refractive index) is commonly used to detect and quantify aggregates present in a protein solution. However, concerns about potential removal of large aggregates by the SEC column as well as sample alteration due to dilution and solvent exchange call for methods orthogonal to SEC. In this scientific poster, we discuss the necessity and benefits of using orthogonal light scattering approaches through a case study of two different lots of Pierce BSA.