AN1609: Drug-Antibody Ratio Analysis in ADCs


Drug-Antibody Ratio (DAR) is a critical attribute of antibody-drug conjugates (ADCs). DAR may often be determined quickly and effectively by combining SEC with three online detectors: MALS, UV and dRI. We present here a method for DAR determination based on SEC-MALS in conjunction with UV absorption and differential refractive index (dRI) detection, which overcomes these limitations for many ADCs.