AN3010: Analysis of hetero-oligomers with CG-MALS

Molecules A and B may bind with different stoichiometries, at different molar ratios of A and B. Adding to this complexity, A and B may also self-associate into homo-oligomers. Here we discuss a method that disentangles all these possibilities in a single experimental run using multi-angle light-scattering.

Composition-gradient multi-angle light scattering (CG-MALS) provides easily interpreted data for complicated macromolecular interactions. Here we show how a simple protocol provides information-rich data with a completion time of a few hours.