AN4003: Influence of heating rates on the thermostability characterization of antibodies by DLS and SLS

Thermostability screening by means of a temperature ramp is a common approach to assessing the aggregation propensity of different candidate molecules and formulation conditions. Such screening is usually performed in a high-throughput manner using dynamic light scattering (DLS), intrinsic fluorescence and/or other techniques to quantify key attributes like the melting temperature (Tm) or the onset temperature of aggregation (Tagg). However, the rate of temperature ramp, and ultimately assay throughput, impact the end results. This application note uses dynamic and static light scattering to probe the speed limitations on thermostability screening.