Competitive Binding Between Thrombin, Antithrombin III, and a Monoclonal Anti-Thrombin Antibody

Multi-angle light scattering (MALS) is a powerful tool for quantifying multiple types of protein-protein interactions. In this application note, we probe the interactions between three proteins: human thrombin-α (Thr), antithrombin III (AT), and an anti-thrombin monoclonal antibody to evaluate whether the antibody can recognize AT-inactivated Thr.