Determination of the Stoichiometry of Protein-Protein Complexes

Domain antibodies (dAbs, 12-14kDa) are small molecules comprising Vh or Vk domains of human IgG molecules, which are capable of binding to various antigens. In this application note, we assess the stoichiometry of interaction between dAbs and their corresponding antigen–in this instance, human serum albumin (HSA), using SEC-MALS.