High Molar Mass Polysaccharides: Comparison of SEC-MALS and FFF-MALS [poster]

High-Molar Mass Polysaccharide

Native alternan is a high molar mass polysaccharide produced by some strains of the bacterium Lueconostoc mesenteroides. The polysaccharide can be purified from cell-free supernatant by several rounds of alcohol precipitation. In this study we used the Wyatt Eclipse Field Flow Fractionation System to fractionate the polysaccharide and multi-angle light scattering (MALS) coupled with a refractive index detector to measure the molar mass and size of native alternan and compared these results to traditional SEC-MALS. The results show that native alternan was highly polydisperse, with a weight average molar mass of 50 million Da and an RMS radius of 45 nm. Learn more by viewing our scientific poster.