Kinetics of Covalent Thrombin-Antithrombin Association

Antithrombin III forms a covalent bond with the catalytic serine of several proteases involved in the coagulation cascade, and the rate of this reaction can increase up to 1000x in the presence of certain macromolecules. In this application note, we determine the second-order rate constant for the covalent association of thrombin-α (Thr) and antithrombin III (AT) by Composition Gradient Multi-Angle Light Scattering (CG-MALS).