AN3008: Metacomplex Formation & Affinity of Multivalent Binding Partners

Multivalent Binding

Multivalent binding plays a crucial role in protein-protein interactions, including cell signaling pathways, immunological responses, and self-assembly of structural elements. However, standard analytical techniques for measuring macromolecule interactions require one binding partner to be immobilized on a surface, leading to errors when both binding partners are multivalent. Composition-gradient multi-angle light scattering (CG-MALS) is unique in its ability to quantify these interactions to yield affinity and stoichiometry of complexes forming in solution.