AN3009: Self-Associating Antibody Solutions at High Concentrations
The characterization of therapeutic protein formulations is essential for the development of novel biopharmaceuticals. In particular, solution viscosity and colloidal stability may be adversely impacted by attractive self-interactions among protein molecules. In this study, we quantified the self-association affinity and stoichiometry of three antibody formulations (mAbs A, B, and C) in their corresponding formulation buffers using composition-gradient multi-angle light scattering (CG-MALS) automated by the Wyatt Calypso®.