Separation of GCSF using AF4

Separation of GCSF

Recombinant granulocyte colony stimulating factor (rh-GCSF, 18.8kDa) is a cytokine of remarkable therapeutic relevance that has a strong tendency to aggregate. In this application note, we develop an Asymmetrical Flow Field Flow Fractionation (AF4) method that provides separation and quantification of the monomer’s soluble and insoluble aggregates as well as the determination of the molecular weights (MW).