AN3002: Understanding Antibody and Viral Glycoprotein Interactions

Viral glycoproteins are often the only antigen found on the viral surface and, as such, are key targets for neutralizing antibodies. Analyzing the type and affinity of the different molecular interactions that can occur is essential for understanding what makes a good neutralizing antibody and how these interactions can be utilized in post-exposure treatments as well as vaccine development. To this end, we used size-exclusion chromatography coupled with multi-angle light scattering (SEC-MALS) to determine the molar mass and native oligomeric state of a viral glycoprotein (vGP), and composition-gradient light scattering (CG-MALS) to determine the stoichiometry and affinity of the glycoprotein:antibody interaction.