AN2614: Virus-like particle characterization using FFF-MALS

Virus-like particles (VLP) are of growing interest in the pharmaceutical sciences. In quality assurance there is a great need for techniques that characterize different VLP fractions (fragments, monomers, dimers, trimers, and aggregates). We have recently demonstrated that the separation and subsequent quantification of different VLP species is possible by field-flow fractionation combined with multi-angle light scattering (FFF-MALS). Common disadvantages of this technique, like long equilibration and analysis times, the need for high sample amounts and large eluent volumes, are overcome through the use of short channel geometries.