Absolute Biophysical Characterization with Multi-Angle and Dynamic Light Scattering

Vivianna Day

Presented by: Vivianna Day, Applications Scientist and Product Manager, Wyatt Technology
Presented Live: June 6, 2017

Traditional size exclusion chromatography (SEC) with UV or refractive index (RI) detection have several limitations that can significantly affect the scientific outcome. Chromatography system with one detector is limited to measure the relative molecular weight of molecules that have similar chemical properties and conformation of reference proteins or polymers that were used to calibrate the column. Multi-angle light scattering (MALS) and dynamic light scattering (DLS) detectors in-line of SEC-UV or RI are essential instruments that resolve those inherent deficiencies by providing the first-principles characterization of molecular weight and size, independent of column calibration. A SEC-MALS-RI or UV with DLS system extends the characterization range of biomolecules to glycoproteins, PEGylated proteins, hydrophobic proteins, biopolymers, and other biomolecules.

This webinar will present the benefits of SEC-MALS and DLS by measuring absolute molecular weight, size, and composition of these species. We will present key analytical methods with the latest MALS, DLS and RI instrumentation that overcome the limitations and uncertainties of standard SEC.

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