Wyatt Technology

Advances in online viscometry: extending the limits of detection for intrinsic viscosity and hydrodynamic radius


Presented Live: October 19, 2016

Speakers:  Dr. Stepan Podzimek, Department Head Analytical and Physical Chemistry, SYNPO
Dr. Steven Trainoff, Chief Scientist, Wyatt Technology Corporation

Online differential viscometers are commonly coupled to gel permeation chromatography (GPC) for molar mass determination via the Mark-Houwink-Sakurada relation, or alternatively the universal calibration technique. However, viscometry’s real strength is realized when coupled with multi-angle light scattering (MALS) to characterize size, shape, branching, and aggregation of polymers and biomolecules, providing deeper insight into macromolecular properties.

New developments in the technology of differential viscometers extend their capabilities to the realm of compact molecules such as proteins. Improved sensor technology in conjunction with a patented thermal balancing system allows for dramatically improved sensitivity and near immunity to pump pulses. We describe a new differential viscometer, incorporating these advances and others, that measures hydrodynamic radius with sensitivity exceeding that of online dynamic light scattering, especially for globular proteins and other compact macromolecules.




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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

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