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Applications of Analytical Light Scattering in a Biophysics Core Facility

 

Presented Live: December 5, 2017

Speakers: Ewa Folta-Stogniew, Ph.D.

Static light scattering (SLS) is an analytical technique for determination of molar masses (molecular weights) and radii of gyration of macromolecules in solution. Measurement of molar mass from an SLS experiment determines the oligomeric state and thus provides association stoichiometry for biological macromolecules such as native and modified proteins and their complexes.
This webcast presents several case studies of the application of size exclusion chromatography (SEC) coupled with multi-angle (static) light scattering (MALS) in analysis of protein structure-function relationship, carried out at the Biophysics Resource of Keck Biotechnology Research Laboratory at Yale University School of Medicine. The examples include studies of heparin-induced dimerization of anaplastic lymphoma kinase (ALK), determination of stoichiometry of nucleic-acid repressor complex involved in regulation of transcription of the c-myc oncogene, deciphering the pathway of oligomerization and higher-order assembly of PopZ polar scaffold protein that supports asymmetric cell division, and re-examination of the role of amino-terminus in dimerization of SecA protein.

 

  Webinar Q & A

 

 

   

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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