Wyatt Technology

Asymmetric Flow Field Flow Fractionation (AF4): A Powerful Alternative to SEC


September 20th 2016, 10 a.m.; duration approximately 30 min

Speaker: Dr. Roger Scherrers, Wyatt Technology Europe GmbH

The topic:

Modern analytics have to face growing challenges. The separation and characterization of therapeutic proteins, for instance, or the analysis of nano-particles and (bio-) polymers become more and more important.
This webinar will introduce field flow fractionation in its theoretical and practical aspects. Moreover, application examples will vividly demonstrate how easy-to-use and efficient this technique works. AF4 can meet all the challenges during separation, purification and analysis of macromolecules. You will learn how easy even difficult samples can be separated fast and efficiently.




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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

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