Wyatt Technology

Characterizing Molecular Structure in Synthetic and Natural Polymers by Multi-Angle Light Scattering


Presented Live: November 22, 2016

Speaker: Dr. Stepan Podzimek, University of Pardubice

Nowadays, in the majority of polymer characterization the standard-calibrated SEC is the employed method. However, as SEC itself is not an absolute technique, it typically provides incorrect information about the molar mass distribution and no additional information about the molecular structure. Especially branched and/or ultra-high molar mass molecules have been shown to exhibit abnormal elution behavior which leads to wrong description of molar mass distribution. In this webinar the principles of detection and characterization of polymers by multi-angle light scattering (MALS) coupled with SEC will be discussed. It will be elucidated how SEC failures can be overcome using the absolute method of MALS. Combination of SEC with a MALS detector also enables to determine the branching distribution and calculate average branching ratios. Moreover, the attendants will learn about the advantages of field flow fractionation for the separation of large and branched macromolecules; and also how combination of SEC with a MALS detector and an online viscometer can provide fundamental description of polymer properties and deep insight into the polymer structure.




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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.

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