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Characterizing Protein-Nucleic Acid Interactions by Light Scattering

 

Presented Live: August 21, 2018

Speakers: Sophia Kenrick, Ph.D., Wyatt Technology

Harnessing the interactions between DNA, RNA, and proteins holds much promise for detecting biomarkers, diagnosing disease, and improving cancer-targeting therapeutics. Quantifying these interactions is essential for understanding and controlling their biomolecular mechanisms. Multi-angle light scattering (MALS) is a powerful tool for directly measuring molar masses of proteins, nucleic acids, and complexes in solution without fluorescent or radio labeling.
In this webcast, an expert will present two complementary light-scattering techniques for determining the stoichiometry and affinity of interactions between proteins and nucleic acids:

  • Size-exclusion chromatography coupled with MALS, ultraviolet, and differential refractive index detection (SEC–MALS) analyzes each of the species present in a solution of macromolecules
  • Composition-gradient MALS (CG–MALS) quantifies the binding affinity and stoichiometry of biomolecular complexes, including multi-step reactions, label-free, and immobilization-free

Application examples will focus on protein-DNA and protein-RNA complexes.

 

  Webinar Q & A

 

 

   

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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