Characterizing Protein-Nucleic Acid Interactions by Light Scattering

Sophia Kendrick

Presented by: Sophia Kenrick, Ph.D., Wyatt Technology
Presented Live: August 21, 2018

Harnessing the interactions between DNA, RNA and proteins holds much promise for detecting biomarkers, diagnosing disease and improving cancer-targeting therapeutics. Quantifying these interactions is essential for understanding and controlling their biomolecular mechanisms. Multi-angle light scattering (MALS) is a powerful tool for directly measuring molar masses of proteins, nucleic acids and complexes in solution without fluorescent or radio labeling.

In this webcast, an expert will present two complementary light-scattering techniques for determining the stoichiometry and affinity of interactions between proteins and nucleic acids:

  • Size-exclusion chromatography coupled with MALS, ultraviolet, and differential refractive index detection (SEC–MALS) analyzes each of the species present in a solution of macromolecules
  • Composition-gradient MALS (CG–MALS) quantifies the binding affinity and stoichiometry of biomolecular complexes, including multi-step reactions, label-free, and immobilization-free

Application examples will focus on protein-DNA and protein-RNA complexes.

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