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Development of a New Online Micro-Viscometer for APC and UHPLC Applications

 

Presented Live: June 19, 2018

Speakers: Steven Trainoff, Ph.D. and Chris Deng, Ph.D., Wyatt Technology

Differential viscometers are commonly used with GPC to determine the intrinsic viscosity of polymers, from which molar mass can be derived under certain assumptions. However, the real strength of differential viscometry is realized when coupled with size-exclusion chromatography and online multi-angle light scattering (SEC-MALS-IV) to measure, from first principles, both molar mass and hydrodynamic (viscometric) radius. SEC-MALS-IV fully characterizes macromolecules in terms of size, conformation, branching, and aggregation.
In this webcast, we will present novel, low-volume, differential-viscometry sensor technology. This new system provides dramatically improved sensitivity and near immunity to pump pulses, as well as extremely low dispersion, compared to conventional differential viscometers. Instruments based on the technology are particularly appropriate for UHPLC and APC applications when combined with low-volume MALS, UV, and differential refractive index detectors.

 

  Webinar Q & A

 

 

   

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Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution.

Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity.



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