Expanding the Characterization Toolkit with FFF-MALS: Developments, Techniques, and Applications

Presented by: Nemal Gobalasingham, Ph.D., Wyatt Technology
Presented Live: June 15, 2021

One of the primary challenges for reliable and accurate characterization of biotherapeutics, nanoparticles, polymers, and proteins is effective analytical separation and detection. This is especially true for inherently polydisperse complexes, and those with aggregates or branched constituents. This webinar explores the latest advances in field-flow fractionation (FFF) coupled with multi-angle light scattering (MALS) to demonstrate how FFF-MALS—in a single-fluid pathway—enables highly tunable separations and robust characterization, while overcoming the inherent limitations of other techniques.

The case studies presented illustrate a broad suite of measurements afforded by FFF-MALS, including multi-detector analysis, high-resolution size distributions from 1–1000 nm, quantitative particle concentration, and zeta potential distributions. Learn how these techniques are applied to a variety of biotherapeutics and functionally relevant polymers, proteins, and complexes. New capabilities presented will include sensitivity enhancements resulting from dilution control and in-silico method development.

Q&A Webinar Q & A