Experimental methodology in a core facility: Light scattering for preliminary experiments and final answers

Presented by: Peter Randolph, Ph.D., Florida State University and Landry Bennett, Ph.D., Wyatt Technology
Presented Live: June 21, 2022

As a core analytical facility, we are approached by users seeking our services with a large variety of samples. They usually have specific experiments/equipment in mind, frequently desiring to use the most intensive experimental techniques (in terms of sample, time, and money), when a simpler technique could give them the answers they require at a fraction of the cost. Often our recommended techniques include preliminary analysis with dynamic light scattering (DLS), followed by multi-angle light scattering (MALS), instead of analytical ultracentrifugation (AUC) or other high-maintenance biophysical techniques.

In this webinar expert speakers explain, with a few examples, the methodology they have developed to save users time, sample, and money by prioritizing certain techniques for preliminary experiments. Many times the results from these ‘preliminary’ experiments are sufficient to provide the answers to the questions our researchers are asking.